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Three hours after plating the cells, we imaged the sample using a fluorescence scanner.
The blots were analyzed using a fluorescence scanner.
The immobilized ssDNA is then imaged using a fluorescence scanner.
Fc (1 μg) was separated by SDS-PAGE, and fluorescence was detected using a fluorescence scanner.
Streptavidin-AlexaFluor647 fluorescence was imaged using a fluorescence scanner (Typhoon, Amersham).
The gels are scanned using a fluorescence scanner, which specifically measures the emission wavelength of each Cy-dye.
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Following a stringent wash to remove non-covalent substrate-modifier interactions, the PTM-conjugated substrates are then detected using 'binders' (e.g. antibodies or streptavidin) labeled with fluorescent dyes and the protein microarrays analyzed using a fluorescence slide scanner.
In these experiments, amine-modified C strands are arrayed on the surfaces of glass slides, and the reaction of S with C produces a fluorescent IR complex that is anchored to the slide surface and can be detected using a fluorescence microarray scanner.
Gels were then scanned for fluorescence with λex/λem 635/670 nm for Cy5-NHS and 532/580 nm for Cy3-NHS using a fluorescence image scanner FLA-70000, Fujifilm Life Science) and silver-stained as described previously [28].
Immunofluorescence-stained slides were visualized using a fluorescence microscope with a Slidebook or Aperio slide scanner.
Fluorescence was read using a Typhoon fluorescence scanner (GE-Healthcare) with excitation at 532 nm and recording using a 580BP30 filter.
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