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Fluorescence measurements were determined immediately using a fluorescence reader (excitation maximum 490 nm; emission maximum: 520 nm).
Fluorescence was read using a fluorescence reader (SpectraMax Gemini XS, Bucher Biotec AG, Switzerland).
Hypericin was washed off, replaced with PBS and intracellular hypericin fluorescence measured using a fluorescence reader (LS50B Perkin-Elmer fluorescence spectrometer, Beaconsfield, UK).
Relative fluorescence units were determined by using a fluorescence reader (Tecan, GeniusPRO, Grödig, Austria) and applying a manual gain of 25.
To measure the level of cellular oxidative stress, cultures were subjected to dihydroethidium (DHE) staining followed by quantification using a fluorescence reader after 24 h of α-synuclein expression as previously described (Büttner et al, 2007).
After the plates were further incubated for 4 hours, the fluorescence of each well was measured at wavelengths of 530/25 and 590/35 nm excitation/emission using a fluorescence reader (BioTek®).
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All fluorescence data were collected using a fluorescence plate reader (SpectraMax M2e Multi-Mode Microplate Reader).
Fluorescence was measured using a fluorescence plate reader (BioTek Synergy HT Multi-Detection Microplate Reader) at wavelengths, excitation 360 nm and emission 460 nm.
At the end of the incubation, resorufin fluorescence was measured using a fluorescence plate reader (BioTek Synergy HT Multi-Detection Microplate Reader) at wavelengths, excitation 540 nm and emission 590 nm.
The fluorescent intensity was measured using a fluorescence plate reader (BIO-TEK FL600, BIO-TEK instruments, Inc., Winooski, USA) after 20 h of incubation at 37°C.
Unbound cells were washed away, and adherent cells were lysed and integrin binding function/expression was detected using a fluorescent DNA-binding dye provided with the integrin binding kit using a fluorescence plate reader (485/530 nm).
More suggestions(13)
using a luminescence reader
using a fluorescence method
using a fluorescence quenching
using a fluorescence microscope
using a fluorescence plate
using a fluorescence spectrophotometer
using a fluorescence polarisation
using a fingerprint reader
using a fluorescence imaging
using a fluorescence assembly
using a fluorescence microscopy
using a fluorescence detection
using a fluorescence spectrometer
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