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Using a fluorescence quenching method, compound 4 could be used to detect the content (100.57%) of copper sulfate pentahydrate (≥ 99%) with a RSD of 1.98%, y = − 0.1127 × + 2.7148, R2 = 0.9703 (Cu2 +: 0.5 8.0 × 10− 5 mol/L), and compounds 4 6 also had utility of calculating the content of anhydrous ferric chloride at a wide range of concentration.
This device measures pO2 by using a fluorescence quenching technique.
The OxyLite probes measure pO2 by using a fluorescence quenching technique (Young et al, 1996).
Divalent metal transport activity was measured using a fluorescence quenching assay in calcein-loaded cells (31).
Metal transport by individual tagged Slc11a2-HA proteins expressed in transfected LLC-PK1 cell clones was carried out using a fluorescence quenching assay, exactly as we have previously described (31).
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Previously, we found a tight association between full-length yeast Asf1 and the H3/H4 dimer using a fluorescence-quenching assay [ 18].
We analyzed the binding of H3/H4 dimers with a C-terminal truncation of H4 at amino acid 94 (H4∆94), using a fluorescence-quenching assay to obtain the KD.
These experiments suggest that the "binding interaction" that we observe quantitatively using a fluorescence emission quenching assay for holo-siderophores and apo-FbpABp is ligand-conformation specific and in fact requires the siderophore to be bound to Fe3+.
We modified the xpt-pbuX guanine riboswitch for use in a fluorescence quenching assay that allowed us to specifically detect and quantify guanine in vitro.
As shown by a fluorescence quenching assay using MIANS-labelled P-glycoprotein (PGP), miltefosine bound to PGP with a K d of approximately 7 μM and inhibited PGP-ATPase activity with an IC 50 of approximately 35 μM.
The DPP-DWCZ EP films were further used as a fluorescence-quenching type sensor for the detection of F− in organic solution with the limit of detection to 4.1 × 10−8 M.
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