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This paper reported an approach to detect DNAzyme cleavage process in real time using a fluorescence probe.
H2O2 was electrocatalytically reduced at the TPMATA-FeCl3/CILE in neutral aqueous solutions via Fenton's mechanism in which hydroxyl radical, OH, was produced, and the radical was detected by using a fluorescence probe.
The nature of the released oxygen was investigated using a fluorescence probe for singlet oxygen: SOSG.
Using a fluorescence probe (DCFH-DA) that primarily detects hydrogen peroxide (H2O2) and peroxynitrite (ONOO−), we found a marked increase in DCF fluorescence in TRAIL-treated R1 cells, compared with WT cells.
By using a fluorescence probe specific for PI3P, we showed that 2-BP caused homotypic fusion of PVC/MVBs rather than fusion of PVC/MVBs to vacuoles (Additional file 1: Figure S6).
By using a fluorescence probe specific for PIP2 [ 41], we showed that 2-BP causes a rapid loss of PIP2 at the PM, which correlates with the complete inhibition of endocytosis by 2-BP pre-treatment.
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The relative binding affinity of phosphopeptides to the Src SH2 domain was tested by competition against a fluorescent probe using a fluorescence polarization (FP) binding assay [11], [17], [18].
(B) GFP-EN2 stably expressing cells treated with EN2 siRNA, negative scrambled siRNA or media only imaged at x20 magnification using a fluorescence microscope and probed with goat anti-EN2 antibody, 48hrs after transfection.
Sodium terephthalate has also been used as a fluorescence probe for assessing the Fenton reaction in extracellular fluid [40] and in vivo using HPLC [41].
The results show that the fluorescence technique can be used to follow the onset of the gel effect in S-DVB coPyrenerization.
N- 4- pyrenyl methyleN- 4- pyrenylyl pheN- 4- pyrenyl (Py-methylene -oxycarbonylrescence probe.
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