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The relative binding affinity of phosphopeptides to the Src SH2 domain was tested by competition against a fluorescent probe using a fluorescence polarization (FP) binding assay [11], [17], [18].
In each group, the VAN concentration in the PBS was measured at designated time points using a fluorescence polarization immunoassay (FPIA) (AxSYM, Abbott Laboratories, Chicago, IL, US).
These compounds were tested for binding to the XIAP-BIR3 and ML-IAP BIR using a fluorescence polarization assay.
Peptides were prepared via solid-phase peptide synthesis and tested for affinity to Src SH2 domain using a fluorescence polarization based assay.
Valproic acid was measured in plasma using a fluorescence polarization immunoassay (FPIA) technology, as previously described [28].
Schust et al. [16] identified another small molecule inhibitor of Stat3, Stattic, using a fluorescence polarization high throughput assay of Stat3 binding.
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The binding activity of A. pilosa on estrogen receptors was examined using a fluorescence polarization-based competitive binding assay.
In this work, we first investigated three small-molecule probes differing in the degree of "methylation state" and their differential bindings to JHDM1A (an H3K36me1/2 demethylase) using a fluorescence polarization-based competition assay.
Poloxin is a synthetic derivative of a well-known PBD antineoplastic inhibitor named thymoquinone, which was identified using a fluorescence polarization-based high-throughput screening assay against a wide library of 20,000 small molecules.
Subsequent work used a fluorescence polarization immunoassay [ 21, 22] but only one documented sequential measurements in a few patients [ 21].
For each potential nonameric binding frame, we synthesized a 12 15 residue peptide containing the region of interest in its natural context, and determined the binding affinity using a competition fluorescence polarization assay (Figure 6a).
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