Proteolytic activities were assessed in 2 h at 25°C by measuring the release of the fluorescent group AMC using a fluorescence plate reader (Fluoroskan Ascent, Thermo Scientific, Waltham, MA, USA) with excitation and emission wavelengths of 380 and 460 nm, respectively.
The fluorescent intensity was measured using a fluorescence plate reader (BIO-TEK FL600, BIO-TEK instruments, Inc., Winooski, USA) after 20 h of incubation at 37°C.
Unbound cells were washed away, and adherent cells were lysed and integrin binding function/expression was detected using a fluorescent DNA-binding dye provided with the integrin binding kit using a fluorescence plate reader (485/530 nm).
The attached cells were labelled with the green fluorescent dye calcein-AM and measured using a fluorescence plate reader at an excitation wavelength of 485 nm and an emission wavelength of 510 nm.
Nonphagocytosed apoptotic PMN or E. coli were washed off, extracellular fluorescence quenched, and phagocytosis determined using a fluorescence plate reader.
Cell lysate (equivalent of 1000 cells) was mixed with TRAPEZE XL reaction mix containing Amplifluor primers, incubated for 30 min at 30 °C and the products of telomerase activity were quantitated using a fluorescence plate reader.
We validated the protease activity assay with three different proteases, i.e., trypsin, caspase 3, and neutrophil elastase, and demonstrated that it can be used to determine protease activity and the effect of inhibitors with small sample volumes in just a few simple steps using a fluorescence plate reader.
All results were measured using a fluorescence plate reader.
DCF fluorescence was quantified after 5 hours (excitation = 485 nm, emission = 530 nm) using a fluorescence plate reader.
Red/green fluorescence was measured using a fluorescence plate reader (excitation/emission = 550/600 nm and excitation/emission = 485/535 nm, respectively).
The amount of Alexa 488 on the surface of the nanospheres was analyzed by the fluorescence intensity using a fluorescence plate reader (Synergy HT; BioTek Instruments, Winooski, VT).
