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Relative fluorescence intensity was recorded over time 3-1200 min) by using a fluorescent plate reader (Thermo, Appliskan) at an excitation wavelength of 485 nm and emission was measured at a wavelength of 530 nm, and fluorescent images were also obtained by using a fluorescence microscopy (ZEISS, Axiovert 200 M).
Images were viewed and photographed using a fluorescence microscopy (DMI 3000B, Leica, Wetzlar, Germany)41.
The morphologies of the treated glioma cells were also studied using a fluorescence microscopy (Zeiss Axio observer Z1, Germany).
Then, by using a fluorescence microscopy method, the group imaged an increase in glycan biosynthesis in the jaw region, pectoral fins, and other organs of the living embryos.
Inclusions were enumerated by visual inspection using a fluorescence microscopy.
After labeling with secondary antibodies conjugated with Alexa 488 or 594 (Invitrogen), images were acquired using a fluorescence microscopy DM6000B (Leica Microsystems, Tokyo, Japan).
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Cells were examined by standard fluorescence microscopy using a fluorescence microscope (Axioplan Microscope, Zeiss, Germany).
To screen compound libraries for molecules inhibiting binding of the outer kinetochore Ndc80 complex (Ndc80, Nuf2, Spc24, Spc25) to MTs, we used a fluorescence microscopy-based in vitro approach (Fig. 1).
Smear microscopy was performed using a conventional fluorescence microscopy after specimen concentration using high-speed centrifugation.
Autophagy was quantified by quantification of EGFP-LC3B puncta per cell using fluorescence microscopy, using an Olympus IX51 fluorescent microscopy system at 60× magnification.
After CTC staining and fixation, samples were examined by using BX51 fluorescence microscopy (Olympus) with a ×10 ocular and ×100 objective (oil).
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using a fluorescence assay
using a fluorescence polarization
using a video microscopy
using a fluorescence method
using a fluorescence quenching
using a fluorescence microscope
using a fluorescence plate
using a fluorescence spectrophotometer
using a fluorescence polarisation
using a fluorescence imaging
using a fluorescence assembly
using a fluorescence detection
using a laser microscopy
using a fluorescence spectrometer
using a fluorescence detector
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