Candidates were confirmed by discriminating homozygote and heterozygote populations using a fluorescence solution method and two colorimetric microarray methods on polycarbonate chips.
Using a fluorescence fluctuation method (fluorescence correlation spectroscopy, FCS), we found that truncation of the EphA2 receptor by the SAM domain leads to increased oligomerization compared to the full length receptor, and causes constitutive activation of EphA2.
Then, by using a fluorescence microscopy method, the group imaged an increase in glycan biosynthesis in the jaw region, pectoral fins, and other organs of the living embryos.
The quantification of Cr3+ by 1 using a fluorescence spectroscopy method was satisfactory in the linear working range below 4 μM, with a detection limit of 3.75×10 9 M for Cr3+ and good tolerance of other metal ions.
Using a fluorescence quenching method, compound 4 could be used to detect the content (100.57%) of copper sulfate pentahydrate (≥ 99%) with a RSD of 1.98%, y = − 0.1127 × + 2.7148, R2 = 0.9703 (Cu2 +: 0.5 8.0 × 10− 5 mol/L), and compounds 4 6 also had utility of calculating the content of anhydrous ferric chloride at a wide range of concentration.
The limits of detection of NADH formed using the chemiluminescence procedure were approx. 1000-fold lower than those established using a fluorescence detection method (results not shown).
Using a fluorescence spectroscopy method they demonstrate that residues outside of the established DBD are not only involved in binding but increase affinity by 5-fold using y-shaped DNA junctions [ 44].
The extracted supernatant was removed and reconstituted in 75 μl of 3 M sodium acetate and a final volume of 50 μl was injected onto a column using a fluorescence detection method.
Andrews and colleagues [ 21] did not find a significant difference in the percentage of CD14+ PBMCs between SSc and healthy controls using a fluorescence microscopy method, whereas an increased population of CD14+ PBMCs in SSc patients were found by flow cytometry in our study.
