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The action of actin takes place mainly at the periphery of the cell, and we have shown using a fluorescence focus assay that the disruption of the actin cytoskeleton causes a reduction in the number of cells successfully infected by JEV.
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To assess the positivity for phospho-H2AX, the cells with more than 10 foci were counted using a fluorescence microscope (Olympus, Tokyo, Japan).
(B) Fluorescence image of the same cells using a fluorescence filter set for green fluorescent protein.
53BP1 DNA damage foci were counted on images obtained using a fluorescence microscope (Leica DMRXA, Germany); 400-500 cell nuclei were counted per sample.
After washing with PBS, fluorescence was visualized using a fluorescence microscope (Leica Microsystems, Buffalo Grove, IL, USA).
The slides were examined using a fluorescence microscope (Carl Zeiss).
The tissue slides were then examined using a fluorescence microscope.
Images were captured using a fluorescence microscope (Olympus BX51TF, Tokyo, Japan).
Images were visualized using a fluorescence microscope (BZ-9000; Keyence).
Bacillus subtilis colony seen using a fluorescence microscope.
Nonphagocytosed apoptotic PMN or E. coli were washed off, extracellular fluorescence quenched, and phagocytosis determined using a fluorescence plate reader.
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