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The amount of benzopyrene was determined using a fluorescence detector with excitation and emission wavelengths of 297 and 408 nm, respectively.
The chromatograms were monitored by UV absorption using a photodiode array detector MD-20188, JASCorp.rp., Tokyo, Japan) and by fluorescence at excitation and emission wavelengths of 460 and 520 nm, respectively, using a fluorescence detector (FP-920, JASCO Corp., Tokyo, Japan).
Data were acquired for 7000 events with a variation coefficient of less than 5%, and red fluorescence was measured using a fluorescence detector 3 (FL3) on the X-axis.
The intense peak in the chromatogram using a fluorescence detector corresponds to MNBDA formed by oxidation by the DNAzyme.
DiIC12(3) was detected using a fluorescence detector with an excitation wavelength of 550 nm and an emission wavelength of 604 nm.
FITC-BSA was detected using a fluorescence detector with excitation and emission wavelengths set at 490 nm and 520 nm, respectively.
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The drop was taken by 1 ml of acetonitrile solvent compatible high performance liquid chromatography by which the extracts were analyzed using a fluorescence emission detector (Waters 2475).
Gradient elution was performed with acetonitrile (solvent A) and water (solvent B): 0 4 min, 70%% A; 4 5 min, 70 100 % A; 5 9 min, 100%% A; 9 10 min, 100 70 % A; 10 15 min, 70%% A. The flow of dansylated, polyamines was monitored using a fluorescence detector Prostar 363 (excitation at 252 nm, emission at 500 nm).
After precipitation with trichloroacetic acid, thiol groups were derivatized with ammonium 7-fluorobenzo-2-oxa-1,3-diazole-4-sulfonate, followed by separation using HPLC with a fluorescence detector [ 26, 27].
Fluorescence detection (350 nm excitation, 450 nm emission) was performed using a RF10AXL fluorescence detector (Shimadzu).
Figure 5b shows the comparison of fluorescence intensities between the nanomachine groups and the control groups, whereby 10 μl suction from the supernatant was spotted and assayed by a fluorescence detector using a 480-nm-wavelength diode as the excitation source.
More suggestions(15)
using a photomultiplier detector
using a combination detector
using a point detector
using a fluorescence method
using a germanium detector
using a frequency detector
using a prototype detector
using a threshold detector
using a self detector
using a conductivity detector
using a fluorescence plate
using a decorrelating detector
using a repetition detector
using a face detector
using a particle detector
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