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Currently, several high-throughput genotyping platforms using a fluorescence detection system are available in rice (Thomson 2014).
In addition, for high-throughput genotyping using a fluorescence detection system, we also developed Fluidigm SNP genotyping markers.
The limits of detection of NADH formed using the chemiluminescence procedure were approx. 1000-fold lower than those established using a fluorescence detection method (results not shown).
equipped with atmosphere controller set at 37°C and 5%CO2 95%% air using a fluorescence detection with 485 nm excitation and 535 nm emission.
Plasma levels of total (lactone plus carboxylate) irinotecan and metabolite SN-38 were determined by a reversed-phase high-performance liquid chromatography method using a fluorescence detection (Vergniol et al, 2000).
The extracted supernatant was removed and reconstituted in 75 μl of 3 M sodium acetate and a final volume of 50 μl was injected onto a column using a fluorescence detection method.
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Quantitation of TS, TP, ERCC1 and DPD and an internal reference gene (β-actin) was done using a fluorescence based real-time detection method (ABI PRISM 7900 Sequence detection System, Perkin-Elmer (PE Applied Biosystemm, Foster City, CA, USA).
The LOD of the control experiment was 10 PFU mL 1, about the same as obtained using a fluorescence plate reader for detection.
Quantitation of the genes of interest and an internal reference gene (β-actin) was done using a fluorescence based real-time detection method (Mx3000P Real Time PCR System, Stratagene).
For the detection using a fluorescence microscope, 1 μM and 1 mM Pb2+ solution were investigated.
N. benthamiana was selected because this plant allows a good expression of YFP-GAI fusion and easy detection using a fluorescence microscope [ 4].
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