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Using a fluorescence assembly assay, we show that the extracellular ANTXR domain drives PA oligomerization.
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Fluorescence spectra were recorded using a fluorescence spectrophotometer F-45000, Hitachi, Chiyoda-ku, Japan).
Finally, the emitted fluorescence was visualized using a fluorescence microscope (Jenus, China).
Slides were examined using a fluorescence microscope.
Sperm were photographed using a fluorescence microscope.
Fluorescence images were captured using a fluorescence microscope (Keyence, Tokyo, Japan) for analysis.
Cells were processed as described above but fluorescence was analyzed using a fluorescence microplate reader.
Cells were examined by standard fluorescence microscopy using a fluorescence microscope (Axioplan Microscope, Zeiss, Germany).
Fluorescence was measured using a fluorescence microtiter plate reader (Victor 2, Wallac, Perkin-Elmer).
Relative fluorescence intensity was measured using a fluorescence spectrophotometer (Cary Eclipse, Varian, Darmstadt, Germany).
Cellular fluorescence was determined using a fluorescence plate reader (MDC Flexstation).
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