Sentence examples for using a fluctuation assay from inspiring English sources

The growth rate is indicated by doubling time, which was measured using a fluctuation assay.

Mutations generated using a fluctuation assay can, in principle, arise anywhere in the genome; however, in practice these mutations very often arise in a handful of genes known to be important in whatever selective medium was used.

There are two main methods used for generating mutant collections: (1) a fluctuation assay procedure and (2) site-directed mutagenesis.

This chapter describes the proper design of a fluctuation assay, several of the methods used to calculate mutation rates, and ways to evaluate the results statistically.

The accurate determination of mutation rates depends on understanding the strengths and limitations of these methods and how to optimize a fluctuation assay for a given method.

Using the fluctuation assay (Luria and Delbrück 1943), we measured the rate at which each strain produced 5FOA-resistant ura3 mutations.

Antifungal Chemical Compounds Identified Using a Pathogenicity Assay.

Protein concentration was determined using a Pierce BSA assay kit (Thermo Scientific).

Proliferation was assessed using a MTT assay as described earlier43.

The glucose assay was performed using a glucose assay kit (Abcam, ab65333) according to the manufacturer's product protocol.

Samples were quantified using a Picogreen assay on a TBS-380 Mini-Fluorometer.