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Cell cycle profiling was done with at least 15,000 cells using a flow cytometer (Becton Dickinson).
Gating strategy using a flow cytometer technique to identify microglia (Mg) and monocyte/macrophages (MMs) in the post-stroke brain.
The expressions of CD11b, CD11c, CD18, and CD35 were evaluated using a flow cytometer.
Phenotypic analyses of lymphocytes were performed using a flow cytometer.
Afterwards the cells were stained with antibodies and analyzed using a flow cytometer.
Cells were examined by fluorescence-activated cell sorting (FACS) using a flow cytometer (BD LSRFortesa).
Cell cycle phase was detected using a flow cytometer (FACS420, BD Biosciences, San Jose, CA).
Annexin-V PE and PI fluorescences were measured by using a flow cytometer.
These populations can easily be distinguished using a flow cytometer [26].
The cellular uptake of drugs was analyzed using a flow cytometer and FlowJo analysis software.
T-cells were positive selected and counted using a flow cytometer by Astarte Biologicals.
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