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LD spectra were recorded by using a flow Cuvette cell in a Jasco J-720 spectropolarimeter adapted for LD measurements (Jasco Analytical Instruments, Japan).
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UV-visible spectra were acquired on a Shimadzu UV-2600 using a quartz cuvette (Shimadzu Corporation, Kyoto, Japan).
UV/Vis spectra were recorded on a Perkin Elmer Lambda 35 UV/Vis double-beam Spectrophotometer at 25 °C in the wavelength range of 200 800 nm using a quartz cuvette (pathlength of cuvette was 10 mm).
Fluorescence spectra were recorded with a fluorimeter (Photon Technology International) using a quartz cuvette.
Cytosolic fractions from Sf9 cells infected with the respective recombinant baculovirus were investigated at 37°C using a heated cuvette mount.
The constructs were run on a Jasco J-815 spectrometer (Easton, MD) using a quartz cuvette (path length ∼1 mm).
In order to attain the target CO2 concentration in the cuvette, commercially pure CO2 was added to the cuvette during the light phase, compensating for the plants' uptake and leakage using a mass flow controller (5850E; Brooks Instrument, Veenendaal, The Netherlands).
First, a reference signal I0 is taken by using a carefully cleaned cuvette filled with pure water to consider any reflections at the surface of the cuvette.
Absorption spectra were measured with a spectrophotometer (PerkinElmer) using a UV-transparent cuvette (Brandtech).
Binding affinity measurements were performed on an ISS spectrofluorometer (Urbana, IL) using a 3 mm cuvette (see [15]).
Spectra were recorded at 25 °C using a 2 mm cuvette.
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