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The reactant pulses were introduced into the gas stream immediately before the catalyst bed using a fixed volume sample loop.
The electrolyte (5 µL) was dropped on the electrodes using a fixed volume pipette, and the contact angles were measured just after dropping.
Assays ran for 4 hours before collecting and quantifying the cells migrated into the bottom chamber using a fixed volume count flow cytometry protocol (Epics XL, Coulter).
It was obtained by placing 5 μL of 2% preservative free sodium fluorescein (NaFl) to the inferior fornix using a fixed volume micropipette.
The assay was based on the oxidation of non-fluorescent 2', 7'-dichlorofluoroscin diacetate (DCFH-DA) by H2O2 to stable and fluorescent dichlorofluoroescein. H2O2 production was assessed in cells using a fixed volume of 0.5 ml cell suspension (2×106 cells/ml) mixed with 0.5 ml DCFH-DA (20µM) in PBS.
cDNA was synthesized with the miRCURY LNA Universal RT cDNA synthesis kit (Exiqon) using a fixed volume of RNA preparation in each reaction according to the manufacturer's instructions.
Similar(53)
On each sampling day, the spirometers were calibrated in the morning before prehike testing and in the afternoon before posthike testing using a fixed-volume, 3-L syringe.
Correction for blood using a fixed (not fitted) blood volume contribution is possible with graphical Patlak analysis, but this correction biases the outcome parameters as the blood volume varies between populations [22].
Therefore, a fixed volume encompassing ROI was used.
The procedure relies on a novel parametric model of blended wing-body shapes which is used to create a re-entry configuration around a fixed volume.
Using a fixed threshold value for functional lung volume delineation provided unacceptably wide differences of volumes in a significant proportion of patients and much lower correlation with PFT parameters compared to a previously validated manual technique.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com