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Linearity was checked by preparing standard solutions at five different concentration levels of each of FEX and its four related compounds ranging from 0.1-50 0.1-50using a fixed concentration of 30 μg/ml LIS as internal standard.
To understand the electrocatalytic behavior of the working electrodes, cyclic voltammetry (CV) technique was firstly applied in a buffer solution of 0.1 M PBS (pH 7) at a scan rate 50 mVs− 1 on bare GCE, mesoporous α Fe2O3-modified GCE, and mesoporous 1 wt.%Ag/α Fe2O3-modified GCE using andixed concentration of 5 mesoporous.
In order to investigate the influence of ADP-Mg on the oligomerization state of the protein, titration experiments were performed using a fixed concentration of DAPKwt (5 µM) and increasing amounts of ADP-Mg.
The transport activity was measured using a filter-based assay as described by Geertsma et al. [40] in a time dependent way (up to 30 min), using a fixed concentration of 50 µM or 5 mM of substrate.
Similarly, higher platelet aggregation was shown in platelets from MetS pigs compared with platelets from lean controls when using a fixed concentration of collagen and AA as agonist.
B. Dose response curves as in Fig. 4 A, except for THP1 cells using a fixed concentration ratio of 1000 1 HU Aza.
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To further investigate assay sensitivity, we used a fixed concentration of the MIP mixture at 5 fmol/probe in the ligation step, while varying the amount of target DNA.
Accordingly, this study used a fixed concentration of an oxidant, rather than multiple additions of oxidants or of the xanthine oxidase system.
For these diffusion experiments we used a fixed concentration of GRG (4.6 mg/ml) and titrated gal-1 into the glycan solution.
In this experiment, we used a fixed concentration (0.8%) of 33-ϕ-OEG-lipid in the membranes in the presence of a second functionalized lipid with a variable concentration (0 4%).
Kinetic constants were determined using Hanes plots from experiments carried out using a fixed inhibitor concentration of 20 mM xylose at varying PNPX concentrations (0.5 - 4 mM).
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