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The lysates were centrifuged at 15,000 rpm for 20 minutes and the supernatant was removed carefully using a fine syringe to avoid the floating fat content.
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After thawing, they were washed with HEPES buffer and homogenized using a fine-bore syringe.
Periodically (after every 10 days), 5 mL samples were withdrawn with the help of a sterilized syringe and filtered using a fine quality filter paper (Whatman, Cat No. 1001917, England).
For cytological analysis, samples were obtained from 2 different areas using a fine needle (22 G1 1/4", Injex®, São Paulo, Brazil) and a 10 ml syringe (Injex®, São Paulo, Brazil).
The skull was opened with fine scissors, and, rather than using a Hamilton syringe as originally reported (Peter and Gill 1975), we used a fine glass capillary held in a micromanipulator as reported for tadpoles (Ouatas et al. 1998).
Use a fine brush.
Use a fine point sharpie.
Bone marrow was flushed from femurs using a syringe with a fine gauge needle and 3 mL of PBS.
Then fill the back using a syringe with a fine needle (0.40 x 42 mm).
Use a fine-pointed needle.
Peripheral blood was extracted from the heart using a syringe equipped with a fine gauge needle.
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