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The actual testing was performed in plates with 96flat-bottomflat-bottomh, using a final working volume of 200 μL per well.
Precultured cells were inoculated at an OD600 of 0.2 in three to four individual bioreactors (Innova Biosciences, Cambridge, UK) using a final working volume of 1.5 L.
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Metabolism assays: Pyrethroids were dissolved in methanol (HPLC grade, Fisher Scientific) immediately before use at a final working concentration of 0.02 mM.
Aliquots of the lysate were then adjusted to a final working concentration of 0.7 μg/μl using the assay buffer provided with the corresponding ELISA kit.
TSA was dissolved in alcohol initially and then diluted with phosphate-buffered saline (PBS) to a final working concentration of 0.1 μg/μl before use [ 9, 10].
Today's aerospace engineers would be loathe to put something in the air that had not been modeled extensively on computers and in wind tunnels, using flight data obtained using avionics, so that the final working product differs little from the prototype.
Quality also increases in Agile projects because using a working system exposes defects right away instead of leaving them to a final testing phase.
And with a final "work work work - make it work!" he leaves and sends the models in for a fitting.
or for a discrete, identifiable portion of a final work product.
You must use a hard working surface.
This review was conducted using a near-final prototype.
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