Sentence examples for using a final volume from inspiring English sources

Filtrated body fluids were diluted into DMEM to final concentrations ranging from 0.1 to 10% using a final volume of 1 ml.

Culture was also performed in 75-cm2 flasks with 0.2-µm vented caps (Corning) using a final volume of 20 ml per flask.

Culture was performed in 24-well plates with flat-bottom wells and covering lid (Corning, Inc., Corning, NY, USA) using a final volume of 1 ml per well.

Four volumes of each protein solution were mixed with one volume of dye reagent concentrate Bio-Rad Laboratoriess) using a final volume of 1 ml of solution.

Quantitative PCRs were done on an ABI PRISM 7900HT Sequence Detection System (Applied Biosystems) in 96-well plates using a final volume of 20 µL and the following cycle conditions: 50°C for 2 minutes, 95°C for 10 minutes, and then 40 cycles of 15 seconds at 95°C and 1 minute at 60°C.

PCR was performed using a final volume of 20 μL.

Reverse transcription reaction was done using a final reaction volume of 20 μL containing 1 μg of RNA, 2.5 μM of oligo dT 18, 10 U Transcriptor Reverse Transcriptase and 20 U Protector RNase Inhibitor (Roche) according to the manufacturer's instructions.

Precultured cells were inoculated at an OD600 of 0.2 in three to four individual bioreactors (Innova Biosciences, Cambridge, UK) using a final working volume of 1.5 L.

The actual testing was performed in plates with 96flat-bottomflat-bottomh, using a final working volume of 200  μL per well.

A total of 850  µL of plasma from the same sample was also extracted using the COBAS AmpliPrep-total nucleic acid isolation (TNAI) kit on the COBAS AmpliPrep DNA/RNA extractor (Roche Diagnostics) using a final elution volume of 75  µL.

Genomic DNA was extracted using QIAamp blood and tissue kits (Qiagen) according to the manufacturer's instructions, using a final elution volume of 100 μL, and tested by IS1111 qPCR.