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The baseline transmission rate is calculated using a final size relation (see Text S1).
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A final size selection was performed using a 2% agarose E-Gel to obtain a library with a median length of ~230 bp which is within the recommended size range for cluster generation on Illumina's flowcell.
A final size selection was performed using a 2% agarose E-Gel to obtain a library with a median length of ~230 bp which is within the recommended size range for cluster generation on Illumina's flow cell.
R0 was estimated for each data set using either the final size of the epidemic (for serologic data and clinical data with good ascertainment) or its growth rate (for epidemic curves) (42, 43) (Tables 1 and 2).
In order to overcome the problem of the unspecific binding of protein to the dextran resin observed when performing separation at pH ranging from 7.0 to 8.0, a higher value of pH 8.4 and increased salt concentration were used during the final size exclusion chromatography (SEC) separation.
However, unlike other studies, we used the outbreak final size distribution equations derived from branching process theory to calculate the risk for a large Ebola outbreak under the assumptions of immediate and delayed transmission control after an importation.
The final "best" assembly presented here was then assembled using a k-mer size of 55, a pair_num_cutoff of 3, and a map_len value of 32.
Intraoperative measurement of the diameters of the ST, GT and the final ACL graft using a closed-hole sizing block with 0.5-mm increments was made and this diameter was used to estimate tendon CSA.
using a standard sizing gradient.
This must be discussed as a caveat to the use of final size to estimate Ro.
Since doubts have been raised in the literature about the use of final size data for emerging diseases (Drake, 2005), a simulation study was also performed to test identifiability, although a recent study by Blumberg and Lloyd-Smith (Blumberg and Lloyd-Smith, 2013) of joint identifiability of two parameters in a related model is also highly relevant in this context.
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