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The water samples were acidified on site with Baker Instra-analyzed® HCl using a final acid concentration of 0.5%% v/v.
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Additionally, the final detection was performed using a nucleic acid paper-based detection chip, and the results were automatically analyzed by software.
Nucleic acid was extracted using a viral nucleic acid extraction kit (Geneaid, Taiwan) from 200 μL of 10% fecal suspension to a final volume of 50 μL of RNase-free H20.
Using a program like Acid, they can combine their source material into a new song.
The final residue was dissolved in 10 ml of 0.01 N HCl and filtered with a 0.45-µm filter membrane for the quantification of amino acids using an L-8800 aminoacid analyzerid analyzer (Hitachi, Japan).
The police said Los Palillos also used a vat of acid to liquefy two bodies.
Cycloheximide (CHX, Sigma) was used at a final concentration of 50 µg/ml; phosphonacetic acid (PAA, Sigma) was used at a final concentration of 500 µg/ml.
Final deprotection and peptidyl-resin cleavage were conducted using a solvent mixture containing trifluoroacetic acid (TFA)/methylphenylsulfide/water/phenol/1,2-ethanedithiol/triisopropylsilane (81.5/5.0/5.0/5.0/2.5/1.0, by volume).
Use a salicylic-acid spot-treater.
The pH of the final supernatant was detected using a standard pH meter to determine acid formation.
To prepare the secreted protein fraction, bacteria were cultured in LB medium at 37°C for 20 hours and proteins were precipitated from the supernatant using trichloroacetic acid (final concentration: 5%) at 4°C for 16 hours followed by centrifugation for 1 hour at 20,000 g, 4°C.
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