Excitation wavelength was alternated between 340 and 380 nm using a filter wheel incorporated to a xenon lamp system (Lambda LS; Sutter Instruments, Novato, CA).
The excitation wavelength was selected using a Life Technologies monochromator (Omega Optical Inc, Brattleboro, VT, USA) and the emission wavelength using a filter wheel (Sutter Instruments, Novato, CA, USA).
Images were acquired every ∼3s with an extended Hamamatsu Digital Camera C4742-95 (Hamamatsu Photonics, Barcelona, Spain) using a dual filter wheel (Sutter Instrument CO, CA, USA) equipped with 340 and 380 nm, 10nm bandpass filters (Omega optics, Madrid, Spain).
Fluorescence at 510 nm was detected every 5 s with a Retiga-Exi camera (QImaging, Surrey, British Columbia, Canada) during excitation with light of 340 and 380 nm wavelength using a motorized filter wheel (Ludl, Hawthorne, NY, USA).
Fluorescent images were sequentially captured with a Cohu 4900 CCD camera, using an automated filter wheel coupled to a Zeiss Axioplan fluorescence microscope and a CytoVision system.
Three images, corresponding to each fluorochrome and the DAPI counterstain, were sequentially captured with a Cohu 4900 CCD (12-bit grey scale) camera, using an automated filter wheel coupled to a Zeiss Axioplan fluorescence microscope (Zeiss, Oberkochen, Germany) and a CytoVision system with software version 2.7 (Applied Imaging, Santa Clara, CA, USA).
Custom software (AutoIt) commands image acquisition, and switches lamps and filters using an automated filter wheel (Thorlabs FW102B) mated to a modified rotary switch (Allied Electronics).
Images were acquired every 2 sec with an extended Hamamatsu Digital Camera (model C4742-95; Hamamatsu Photonics, Barcelona, Spain) using a dual-filter wheel equipped with 340 and 380 nm, 10-nm bandpass filters.
A filter wheel (96A354, LUDL Electronics) was used to select the transmission/fluorescence signal at the desired wavelength ranges.
First, the white light illumination used for SLIM allows for easy integration of a filter wheel into the setup to perform absorption measurements.
For detection we used interference filters (Semrock, Rochester, NY, USA) mounted in a filter wheel (Sutter Instruments, Novato, CA, USA) and an electron-multiplying charge-coupled device (EMCCD) camera (DU-888E; Andor) controlled by MetaFluor software (Molecular Devices, Downingtown, PA, USA).
