Sentence examples for using a filter plate from inspiring English sources

Murine cytokines were quantified by bead-based immunoassays according to the manufacturer protocol, using a filter plate and a vacuum filtration system for washing steps (LEGENDplexTM Mouse Inflammation Panel kit, BioLegend).

After pretreatment, the solid and liquid fractions were separated by filtration using a filter plate at the T g in the same reactor.

The STAT3 DNA complex was then separated from free probe by using a filter plate.

After incubation, samples were washed 3× with PBST using a filter plate (1.2 μm pore size, Millipore) vacuum apparatus.

Purification with streptavidin sepharose beads and codenaturation of the biotinylated PCR products and the sequencing primer (15 pmol per reaction) were conducted following the PSQ sample preparation guide using a filter plate device.

Fluorescence was monitored using a Glomax Multi+ plate reader using a filter set that allowed excitation at 625 nm and monitoring of emission over 660 720 nm.

The nuclear extracts were collected for STAT3 DNA binding activity using a STAT3 filter plate assay as described under Materials and Methods.

Use a filter.

BAC DNA was then isolated using a modified alkaline method with lysate clarification using Fritted Filter Plate (NUNC, Roskilde, Denmark).

Specific cake resistances of E. coli TOP10 cells under various filtration conditions were next determined using both filter plate designs and shown to be equivalent to those determined using the membrane cell.

DNA was extracted and purified following the protocol of Ivanova et al. [29], using a 96-well filter plate with 1 ml wells and 3.0 µm glass fiber.