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The affinity of DDX3wt and DDX3ΔINS proteins for dsDNA was assessed using a filter binding assay.
GTPγS binding was measured using a filter binding method as described previously (Garcia-Marcos et al., 2010, 2011b).
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To better quantitate the inhibition of WT and mutant 3Dpols by the inhibitors we used a filter binding assay that can be run efficiently in a 96-well format.
They used a filter binding assay and other methods to show that Spot 42 bound very inefficiently and nonproductive to purified 70S ribosomes, and concluded that Spot 42 function is mediated by the RNA itself.
RNA binding by recombinant NS5B RNA polymerase was determined using a filter-binding assay as previously described [26].
The selected population was characterized for binding to NgR using a filter-binding assay similar to that used for the selection.
Samples were then analyzed for radiolabeled product formation using a DE81 filter binding assay.
We confirm that RNA binding of eIF4Af is increased in the presence of silvestrol using an RNA filter binding assay and that this binding is inhibited by hippuristanol (Fig. 2A).
The apparent association constants for the binding of radiolabelled RNAs to ZF proteins were determined using a double-filter binding assay.
We envisioned an overall system in which peptides generated by cell-free expression would be brought to equilibrium with their cognate protein, and bound peptide protein complexes would be separated from the unbound peptide using a double-filter binding assay.
To confirm these results, a filter binding assay was performed using the same complex preparations as for the EMSA but with the weight excess of the protein over the RNA to bind as much RNA as possible to the membrane for the sake of better detection.
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