In-cell ECD fragmentation experiments were performed with an m/z 900-1200 precurselectiontion range using a cathode filament current of 1.2 A and a grid potential of 12 V as previously described (Serpa et al., 2013).
The myth busters proved that you can indeed pick a lock using the filament of an incandescent light bulb and build an electromagnet using household batteries, tape and insulated wire.
Ipsilateral hindpaw mechanosensitivity was assessed by plantar application of von Frey hair filaments using a modification of the Dixon up-and-down method [ 27].
To convert from surface density to fractional occupancy, we used the area occupied by 1 μm of actin filament (0.006 μm), using a value of 370 subunits per micron of filament.
Overlapping boxes 192 px long (2.3 Å/px) were cut from the long filament boxes using a shift of 6 px (97% overlap), generating 64,980 boxes.
41 Mechanical detection thresholds will be tested using a set of von Frey filaments (Opti-hair2-Set, Marstock, Germany).
The innovative solution presented here increases within the range 102 103 the surface interacting with the pumped gas by using an array of filaments, either disordered (felt) or ordered (spiral).
In the current work an oral solid dosage form was created by Fused Deposition Modeling (FDM), using a custom built filament comprised of a water soluble polymer polyvinyl alcohol (PVA), mannitol and hydrochlorothiazide (HCTZ) as model drug and further co-formulated via Hot-Melt Extrusion (HME).
The paw withdrawal threshold in response to a mechanical stimulus was measured every two days using a series of graded von Frey filaments.
The mechanical-detection threshold (MDT) was quantified using a set of 17 von Frey filaments (Aesthesiometer, SOMEDIC, Sweden) at a patient's Pain-Maximum and Pain-Adjacent points, on the back of their right hand, and on the dorsum of their right foot according to the QST protocol [ 38].
