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Functionality of intestinal barrier can be ascertained in flies using a dye permeability assay in which flies are fed a nonabsorbable blue dye (Rera et al., 2011, 2012).
We then quantified embryonic lethality and adult sterility in homozygous backcrossed mutants at both the permissive (15°) and restrictive (26°) temperatures, and quantified the eggshell-defective phenotypes in embryos produced at the restrictive temperature using a dye permeability assay (Table 1).
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We determined Cx43 channel function using a dye spread assay.
Leaks are confirmed using a dye test and then patched.
Mix the dye using a dye brush until the two are evenly blended.
ViroSeq uses a four-dye termination system, while the TrueGene uses a dye primer system.
Or two parts conditioner one part dye if you're using a light dye!
When using a chemical dye, apply a fixative based on the type of fabric being dyed.
Cells were counted using a DNA intercalating dye that fluoresces upon incorporation (CyQuant dye, Invitrogen).
Use a clean dye brush to mix the dye in a disposable plastic bowl.
To test whether full-length recombinant FLAG-SH was functional, we tested its ability to induce membrane permeability using a previously described liposome dye-release assay [11].
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