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The cultures were observed under Axiovert 200M Zeiss fluorescence microscope (Carl Zeiss) using a dual filter for FITC and rhodamine.
Images were acquired every ∼3s with an extended Hamamatsu Digital Camera C4742-95 (Hamamatsu Photonics, Barcelona, Spain) using a dual filter wheel (Sutter Instrument CO, CA, USA) equipped with 340 and 380 nm, 10nm bandpass filters (Omega optics, Madrid, Spain).
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Recursive updates are obtained from a restricted variational Bayesian perspective, using a dual filtering formulation where parameters are allowed to evolve in time.
Differential gene expression was calculated using a dual filtering criteria; (1) an estimation of statistical significance through the Local Pooled Error test (LPE) [64] calculated using S-Plus Array Analyzer with an adjustment for false discovery rate calculation of p value of <0.05 (Benjamini Hochberg) and (2) a fold change (FC) limit of 1.5.
Images were acquired every 2 sec with an extended Hamamatsu Digital Camera (model C4742-95; Hamamatsu Photonics, Barcelona, Spain) using a dual-filter wheel equipped with 340 and 380 nm, 10-nm bandpass filters.
Here, we set out to explore two such markers – CRAE and CRVE – obtained with two different techniques: one using a red-free filter and the other one using a dual wavelength filter used for taking retinal oximetry images and extracting only the information from the green channel.
All signals from GFP- or YFP and RFP-tagged proteins were acquired simultaneously using a Dual View filter system (Optical Insights, NM, USA) at two frames per second.
In this study, titanium aluminium nitride (TiAlN) coatings were deposited using a dual source filtered arc evaporation system with a view to determine the effect of bias voltage increased from −50 to −250 V, the residual stress in the coating as well as the adhesion and cutting tool performance.
To allow the proposed method to be applicable to different temperature and aging conditions, a training-free battery parameter/state estimator is presented based on an equivalent circuit model using a dual extended Kalman filter (DEKF).
This paper describes a form of discrete wavelet transform, which generates complex coefficients by using a dual tree of wavelet filters to obtain their real and imaginary parts.
Fluorescence emission was separated from excitation by using a dual fluorophore beamsplitter FF493/574-Di01 (Semrock) and collected with a dual band emission filter FF01-512/630 (Semrock) using an IMAGO charge-coupled device (CCD) camera (640×480 pixels; TILL Photonics).
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