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Mitochondrial morphology was inspected by fluorescence microscopy using a dsRED filter on a Zeiss Axioskop microscope.
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Fish were submerged in a 1 μg/ml solution of AO (Sigma-Aldrich) and were then anesthetized in 0.017% tricaine and mounted in 3% methylcellulose for visualization using a Leica MZ16F fluorescence stereomicroscope with a dsRed filter.
Three pictures of the seed are acquired at minimum magnification (×0.72) using a charge coupled device (CCD) camera; (i) brightfield, (ii) UV through a dsRed filter and (iii) UV through a GFP filter.
The DsRED fluorescence was observed by separating infiltrated leaf from the plant, 6 days post infiltration, and observed under Leica MZ16-F epifluorescence microscope under a DsRED filter set.
Fluorescence proteins were excited with a mercury metal halide bulb laser, filtered with GFP3 filter system (exciting: 470/40 nm, barrier: 525/50 nm) for GFP fluorescence and a DsRED filter system (exciting: 545/30 nm, barrier: 620/60 nm) for mRFP fluorescence.
Images were reconstructed using a dedicated cardiac filter (CC-filter).
One landmark study isolated the link using a cultural filter.
Using a fuel filter wrench, remove the old fuel filter.
Filter your water water using a tap filter or a pitcher filter.
We quantified the total number of cardiomyocytes in control and TNNT2sp morphant embryos using a cmlc2::DsRed-nuc transgenic zebrafish reporter line [red fluorescent protein (DsRed-nuc) expressed under the control of the cardiac myosin light chain-2 (cmlc-2) promoter] that fluorescently labels the nuclei of differentiated cardiomyocytes (Mably et al., 2003).
We coexpressed the CRY2-Mid2 fusion constructs with CIB1-Ste5 or CIBN-Ste5 and assessed activation of the pathway using a PFUS1-DsRed reporter.
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