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Genomic DNA was isolated from pectoral fin clips using a DNeasy Tissue Kit (Qiagen).
Genomic DNA was extracted from ear tissue using a DNeasy Tissue Kit (Qiagen).
DNA was extracted using a DNeasy tissue kit (Qiagen, Hilden, Germany) following the manufacturer's protocols.
Extractions on recently collected specimens (1993 2005) were done in a common lab, also using a DNeasy Tissue Kit (Qiagen).
DNA extraction from tissue samples was conducted separately using a DNeasy Tissue Kit (Qiagen Japan, Tokyo, Japan).
Total RNA was extracted using an RNeasy Mini Kit (Qiagen, Hilden, Germany) and genomic DNA was isolated using a DNeasy Tissue Kit (Qiagen) according to the manufacturer's recommendations.
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The DNA of each individual was extracted from fresh or silica-dried leaf tissue using a DNeasy Plant Tissue Kit (QIAGEN).
DNA was extracted from 2 mm tissue samples using a DNeasy™ tissue kit (Qiagen Ltd).
Total genomic DNA was extracted from muscle tissue in periopods of six different C. maenas (two from each population) using a DNeasy™ Tissue Kit (Qiagen) and then pooled into a single volume.
H. pylori DNA was isolated using a DNeasy® Tissue Kit (Qiagen; Hilden, Germany) or Amplicor Respiratory Specimen Preparation Kit (Roche Diagnostics GmbH, Mannheim, Germany) according to the manufacturer's protocol.
Genomic DNA was isolated from a M. vitrata specimen (from Burkina Faso; BUR38) [28] using a DNeasy animal tissue kit following the manufacturer's instructions (Qiagen, Valencia, CA).
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