Cellular and humoral immunogenicity in mice was demonstrated using a DNA prime/rFPV boost and vaccinia virus challenge model.
To narrow down the geographical origins of unidentified cadavers, a rapid and simple method to detect JC virus (JCV) genotypes using a DNA chip was developed.
Therefore, EBV seems to be a DNA virus that uses the macroautophagy machinery for its replication.
AAV is a DNA virus that is used for gene therapy and that undergoes limited integration into host genomes.
Most gene therapies target a cell's DNA, using a virus to insert a corrected version of a gene, which then pumps out functional RNA and healthy protein.
The specificity of the real-time PCR assay was evaluated using several DNA viruses including goatpox virus (Accession number: AY077836.1), sheeppox virus (Accession number: AY077833.1), and swine pseudorabies virus (HB-98 vaccine Strain).
About 1 mg of a feces sample was used to extract RNA, using a TIANamp Virus DNA/RNA Kit, and labeled with Cy5 following random amplification.
Microbial nucleic acids were extracted from serum, plasma, throat swabs, nasal lavage, feces, cerebrospinal fluid, and other body fluid using a TIANamp Virus DNA/RNA Kit (TIANGEN Biotech., Beijing, China).
Instead of using a virus to deliver the reprogramming genes, the researchers used a small circular piece of bacterial DNA which can replicate in the cytoplasm.
This is the first anti-cancer efficacy reported using a microRNA regulated oncolytic DNA virus.
