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The latter possibility was evaluated using a DNA template containing the enhancer and promoter in close proximity to each other.
RNA can thus be produced in vitro, i.e. outside the cells, using a DNA template containing the sequence of a specific antigen.
Using a DNA template extracted from PemoNPV-infected shrimp by a viral nucleic acid kit, the detection limit of LAMP was 0.7 fg while that of nested PCR was 70 fg; therefore, the LAMP assay was 100 times more sensitive than nested PCR.
Amplification signals were quantified by the standard curve method using a DNA template series (1.0, 200, 5.0, 10, 20, 40, and 60 ng) on every plate.
For SHAPE analysis, fhlA220 mRNA was in vitro transcribed using a DNA template that was amplified in XL-10 cells using primers 5'-GGAACCGAATTCTAATACGACTCACTATAGGCCTTCGGGCCAAGCAGCGTTACATTCCCATCCACTG-3' and 5'-CCGAGCGGATCCAATATTGAACCGGACCGAAGCCCGATTTGGATC CGGCGAACCGGAthatTGTCCGAGTGATGTCGAACAACCC-3' that includes a structure cassette in the 5' and 3' regions as previously described [29].
Control reactions were performed using a DNA template from a healthy individual.
Similar(47)
HRR uses a DNA template, either in the form of a sister chromatid, homologous chromosome, or repeated sequence, to ensure high-fidelity repair.
In contrast, repair by HR is typically error-free because it uses a DNA template to correct the break.
One μL of genomic DNA from each dilution was used as a DNA template for the LAMP and another 5 μL of genomic DNA was used as a DNA template for the PCR assay.
FTA paper discs used as a DNA template in the PCR proved impractical for testing several pathogens from a single dried CSF spot using single assays.
The TE layer was used as a DNA template to amplify the rDNA sequence with NL1 (5'-gcatatcaataagcggaggaaaag-3') and NL4 (5'-ggtccgtgtttcaagacgg-3') as primers.
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