The PCR products of selective amplifications were separated by capillary electrophoresis using a DNA sequencer (ABI PRISM® 3130 XL Genetic Analyzer, Applied Biosystems, USA).
The RespiFinder-19 analyzes the amplified PCR products by capillary electrophoresis using a DNA analyzer (ABI 3730, Applied Biosystems, Darmstadt, Germany), whereas the RespiFinder-SMART-22 analyzes by melt curve analysis on the RotorGene Q (Qiagen, Hilden, Germany).
The resulting PCR products were analyzed on a Qiaxcel capillary electrophoresis instrument (Qiagen) using a DNA Screening Cartridge (Qiagen).
The nucleotide sequence of Rshtmt was determined using a capillary DNA sequencer 310 (Applied Biosystems) and was deposited in the DNA Data Bank of Japan (DDBJ) database under accession no.
Cloned inserts were sequenced in both directions using the Big DyeR Terminator v3.1 cycle sequencing kit and the sequenced products were analysed using a capillary DNA sequencer (ABI PRISM 3100).
For example, 12 mtgenomes can be sequenced with a chip per day, as opposed to 12 mtgenomes per month, using a 16 capillary DNA sequencer, a 30-fold increase in productivity and data acquisition.
Here, we present a high throughput method for quantitative analysis of plant cell wall polysaccharides using a 96 capillary array DNA sequencer (ABI 3730xl).
After the encoding reaction, only the DCNs regions and not the SNP specific regions are amplified using the universal primers and then SNP genotype is read out using DNA capillary arrays.
Sequencing of the BAC clone was performed by shotgun sequence method using an ABI3700 capillary DNA sequencer (Applied Biosystems).
DNA extraction, microsatellite amplification by Polymerase Chain Reaction (PCR) and genotyping were performed by the laboratory LABOGENA (Jouy-en-Josas, France), using a capillary sequencer (3730 DNA Analyzer; Applied Biosystems, California, USA).
