Using a dual DNA aptamer (CEA aptamer linked to hemin aptamer), capable of rapidly capturing carcinoembryonic antigen (CEA) and hemin, an all-in-one dual-aptasensor with 1,1′-oxalyldiimidazole (ODI) chemiluminescence detection was developed for the early diagnosis of human cancer.
We have demonstrated this concept using two RNA-cleaving deoxyribozymes, each adjoined to a DNA aptamer that binds ATP.
We developed a DNA aptamer AraHH001 the targets mTECs using a cell-SELEX method 44.
TD05, a DNA aptamer against lymphoma Ramos cells, being used as the model, a series of modification strategies were designed and optimized with different positions, numbers and combinations.
However, during the entire selection of a DNA aptamer, a flow cytometry assay and during aptamer-targeted protein purification, RepCell 44 dish was used (CellSeed Inc. Tokyo, Japan).
A DNA aptamer with affinity and specificity for human osteopontin (OPN), a potential breast cancer biomarker, was selected using the SELEX process, considering its homology rate and the stability of its secondary structures.
To address these issues using a new approach, we performed SELEX against IL-1α and successfully isolated a high-affinity (K d = 7.3 nM) IL-1α-specific modified aptamer designated SL1067, which is a DNA aptamer that contains 2-naphthylmethyl substitutions at the 5-position of deoxyuridine (2Nap-dU).
Porciani, D. et al. Two interconvertible folds modulate the activity of a DNA aptamer against transferrin receptor.
Second, displaying a DNA aptamer specific to prostate-specific membrane antigen (PSMA) allows the VLPs to specifically target PSMA+ prostate cancer cells, our model disease target.
