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The air sample genomic DNA prepared using a DNA Purification Kit (PUREGENE) still contained black particles from the air filters, which were found to be inhibitory to PCR and restriction enzyme digestion.
DNA was purified using a DNA purification kit (Qiagen).
Where appropriate, DNA was purified using a DNA purification and concentrator kit (Zymo Research).
DNA was extracted from cells using a DNA purification kit (5 PRIME ArchivePure, Fisher Scientific).
The DNA extraction and purification was performed using a DNA purification column (QIAmp DNA Mini Kit, Qiagen, Valencia, CA).
All PCR products were purified using a DNA purification system (Promega) according to the manufacturer's protocol.
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PCR products were purified using a DNA Fragment Purification Kit (Takara, Japan), and then barcoded and pooled to construct the sequencing library.
Since TRF2 can interact with the MRN components, we tested whether MRE11 could be recruited to DS in vitro using a DNA affinity purification assay (Fig. 1A).
Bands of the expected size (~500 bp) were excised from the agarose gel and purified using a DNA gel purification kit (Sangon, Shanghai, China).
PCR products were purified using a DNA gel purification kit, sequenced using the dideoxy four-color fluorescence method on an automatic sequencer (ABI, U.S ., and compared using GenBank data to confirm the mutation sites.
The amplification products were purified using a DNA and Gel band Purification kit (GFX TM PCR kit, Amersham Biosciences USA).
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