Their integrity was assessed using a DNA 1000 LabChip on an Agilent 2100 Bioanalyzer (Santa Clara, CA, USA).
However, five loci could not be sequenced (bruce06, bruce07, bruce09, bruce30, bruce55) and their length was determined by comparing the PCR product bands with those of B. melitensis 16MT on an agarose gel, and by the Agilent 2100 Bioanalyzer using a DNA 1000 LabChip kit (Agilent Technologies, Waldbronn, Germany) followed by comparison with the results of De Santis et al. 12.
An Agilent Bioanalyzer evaluated the cDNA profile using a DNA High Sensitivity LabChip kit.
Libraries were quantified by qPCR using the KAPA Library Quantification Kit for Illumina Libraries (KapaBiosystems, Wilmington, MA, USA) and library profiles were assessed using a DNA High Sensitivity LabChip kit on an Agilent Bioanalyzer (Agilent Technologies, Santa Clara, CA, USA).
After bead purification, PCR product quantification and size distribution was determined using a DNA assay (GX 1K/12K/High Sensitivity Assay Labchip; catalog no. 760517; Caliper, Hopkinton, MA, USA).
Amplicons were separated using a Diversilab® DNA LabChip kit with microfluidic technology, as described previously [ 18].
The DNA fragments were visualized using an Agilent 2100 BioAnalyzer on a DNA LabChip 7500 with an optimal size of 5 kb.
DNA fragmentation was viewed using the Agilent 2100 BioAnalyzer on a DNA labchip 7500 with an optimal size of 4.9 kb.
The quality of the products was assessed on a Bioanalyzer 2100 (Agilent, Palo Alto, CA, USA) using a DNA1000 LabChip (Agilent).
Eight kb fragmentations were evaluated using a DNA12000 LabChip kit on an Agilent Bioanalyzer and quantified using a Qubit Fluorometer (Life Technologies, Carlsbad, CA, USA).
