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Purified sequencing reactions were electrophoretically analyzed using a DNA Analyzer 3730xl (Applied Biosystems).
The RespiFinder-19 analyzes the amplified PCR products by capillary electrophoresis using a DNA analyzer (ABI 3730, Applied Biosystems, Darmstadt, Germany), whereas the RespiFinder-SMART-22 analyzes by melt curve analysis on the RotorGene Q (Qiagen, Hilden, Germany).
Resuspended products were analysed using a DNA analyzer 3730xl (Applied Biosystems) and the resulting sequence traces were analysed using Sequence Analysis 5.2.2 (Applied Biosystems).
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The libraries were analyzed using a DNA Fragment Analyzer (Advanced Analytical Technologies, Ames, IA) and quantified by real-time PCR using the KAPA Library Quant Kit (KAPA Biosystems, MA).
All T7 DNA was sequenced per Novagen's suggested protocol using a DNA sequencer (Applied Biosystems, 3730XL DNA Analyzer, UM DNA Core).
Resultant plasmid DNA was sequenced using a 3730 DNA Analyzer (Applied Biosystems, Foster city, CA, USA) at the Plant-Microbe Genomics FaciliTheat The Ohio State University.
Samples were resuspended in 10 μl of Ultrapure water and analyzed using a 3730XL DNA analyzer (Applied Biosystems).
DNA sequencing was performed by using a 3730 DNA Analyzer (Applied Biosystems, Foster City, CA, USA).
All these tests use a DNA sequence analyzer to generate profiles of the serum protein N-glycans of liver disease patients.
Randomly picked clones were sequenced using a ABI3730 DNA Analyzer (Applied Biosystems, Foster City, CA, U.S). and the sequences were assembled and analyzed using DNAStar software (DNASTAR Inc., Madison, WI. U.S).
We separated PCR products using a 3730 DNA Analyzer (Applied Biosystems Inc).
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