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Nucleotide exchange can also be chemically induced using a divalent metal ion chelator-like EDTA that could chelate the Mg2+ at the active site leading to nucleotide exchange.
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In our study here, EDTA used in concentrations of 1 and 10 mM as a divalent metal ion chelator showed no effect on activity emphasizing that divalent metal ion presence does not seem to be essential for activity.
Ytterbium, like europium, is a divalent metal.
GH4 enzymes are unique in their requirement for NAD(H) and a divalent metal for activity.
In this case, phosphorylation of the substrate creates a divalent metal ion binding site.
Pure fermium metal has not been prepared, but volatility studies have been carried out with alloys that are dilute solutions of fermium metal in samarium and ytterbium, indicating that fermium appears to be a divalent metal.
The enzyme required thiamine diphosphate and a divalent metal ion, either Mg2+ or Mn2+, for enzymatic activity.
Tetracycline (TC), minocycline (MIN) and tigecycline (TIG) share a divalent metal ion binding site essential for mediating their antibiotic function.
All three drugs selectively inhibit the strand transfer reaction by chelating a divalent metal ion in the enzyme active site.
Our results showed that RBUP was a divalent metal ion-dependent, nonspecific DNase.
These results suggested that the active catalytic domain of the Bgl.bli1 protein contains a divalent metal ion binding site.
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