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Phenotypes were scored in live wild-type or morpholino-injected embryos using a dissection stereomicroscope.
All organs were examined for AL3 stages of Gnathostoma spp. by using a dissection stereomicroscope (magnification ×4 7) then placed in a commercial grade food blender in a 5% pepsin hydrochloric acid solution and macerated.
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We describe a whole-mount staining protocol where erythrocytes were stained with DAB/H2O2 and the tissue is subsequently clarified with BBBA, which made it possible to visualize the entire neovasculature using a regular dissection stereomicroscope.
All digested samples were centrifuged at 3,000 g; solid residue was then rinsed in phosphate-buffered solution, and residue was examined for AL3 with a dissection stereomicroscope (magnification ×4 7).
was performed, embryos were dissected and images captured using a fluorescence stereomicroscope.
The use-wear analyses included the observation of the cutting edges, which was made using a binocular stereomicroscope Motic SMZ171.
The movements were carefully checked using a light stereomicroscope.
We aligned the wire horizontally and measured the deflections using a 60× stereomicroscope.
However, when imaging using a standard stereomicroscope only surface staining can be detected.
After 48-72 hr, we scored the progeny of injected worms using a fluorescent stereomicroscope (Olympus S2x16).
Images of perithecia were captured using a SZX9 stereomicroscope (Olympus) and a digital camera.
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