Sentence examples for using a direct lysis from inspiring English sources
Nucleic acid was extracted from each sample using a direct lysis protocol involving bead beating (Noll et al. 2005).
We have optimized a direct lysis method for qPCR analysis of limited numbers of cells [ 25] and used this method to validate the molecular identity of the cell populations identified by FACS.
DNA was extracted from 100g of soil using direct lysis with glass beads and SDS followed by potassium acetate precipitation, polyethylene glycol precipitation, phenol extraction and isopropanol precipitation.
Extraction of nucleic acids was carried out using a Lysis Buffer for Microorganism to Direct PCR kit (TaKaRa Bio Inc., Tokyo, Japan), supplemented by the TaKaRa MiniBEST Universal Genomic DNA Extraction Kit Ver.5.0 (TaKaRa), according to the manufacturer's instructions.
We compared protocols using a separate lysis buffer with cell capture directly in RT PA reagent.
Protein lysates were prepared using a TCA lysis buffer [49].
Cells were lysed using a RIPA lysis buffer (Beyotime).
Erythrocytes were lysed using a standard lysis buffer.
Protein lysates were prepared using a cell lysis kit (Bio-Rad, Hercules, CA, USA).
Cells were lysed using a periplasmic lysis protocol adapted from Peripreps Periplasting Kit (EpiCentre).
