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In addition, the NGS SNP assay and three CE-based assays (two STR and one InDel assay) were tested using a dilution series consisting of 0.5 ng, 0.1 ng, and 0.05 ng non-degraded DNA.
Colony forming units (CFU) were quantitated using a dilution series plated overnight at 37°C.
The similarities of linearity of primers for each target miRNA were confirmed by using a dilution series of synthetic miRNAs.
Briefly, a tissue array was deparaffinized in xylene and rehydrated using a dilution series of 100 %, 95 and 75% and 75%.
The limit of detection of the Taqman assay was assessed using a dilution series of DNA from isolates of B. pseudomallei and B. mallei.
Fig. 2B shows the Real Time PCR results using a dilution series of the cell lysate and of the standard as templates; all curves have virtually ideal slopes and offsets.
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To establish the initial gradient of functional group richness we used a dilution series (100%, no dilution; 75%, diluted with 25% filtered rock pool water; 50%, diluted with 50% filtered rock pool water; 25%, diluted with 75% rock pool water).
To test the detection performance of MixHMM on real tumor samples with known proportion of 'FM' cells, we used a dilution series of breast cancer cell lines studied by [12].
Primers used for quantification of gene expression levels were tested for amplification efficiency prior to use with a dilution series of an arbitrary cDNA sample.
Primers that were used for quantification of gene expression levels were tested for amplification efficiency prior to use with a dilution series of an arbitrary cDNA sample.
A pool comprising similar amounts of all cDNA samples was used in a dilution series to determine primer efficiencies.
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