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To assess lipid production in continuous cultures, yeasts were grown in a chemostat for over 400 h using a dilution rate of 0.01 h−1.
Steady-state aerobic chemostat cultures were grown at 30 ˚C in 1.2 L bioreactors (DASGIP, Germany) with working volume of 0.5 L using a dilution rate of 0.10 (±0.005) h-1.
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HRad18 antibody was purchased from Santa Cruz and used at a dilution rate of 1∶500 for Western blotting.
In general, the highest inhibition zones were observed when the samples were applied undiluted, except for honey A against S. sonnei, which showed the greatest susceptibly when the honey was used at a dilution rate of 1/1 (50.0% v/v).
Optimal cDNA dilutions and relative concentrations were determined using a dilution series per gene.
The maximum PHB content, 18.34 mg/gDW, was obtained when the mixed substrate was used in the feed at a dilution rate of 0.05 h-1.
In all immunostainings under the universal conditions established here, we used a fixed dilution rate of 1∶500 for all the antibodies applied.
In brief, the chemostat cultivations were nitrogen limited using fructose as a carbon source with a dilution rate of 0.04 h-1.
Therefore, in this experiment, a dilution rate of 0.01 h−1 was used.
The non-lipid cell mass yield increased over the entire dilution rate ranges and reached 0.30 g/g at a dilution rate of 0.14 h−1 (Fig. 2), indicating that more acetate was used for cell growth at higher dilution rates.
Fermentation conditions used a stirrer speed of 800 rpm, with pH of 5.0 (maintained by automatic addition of 2 N potassium hydroxide) and a dilution rate of 0.05 hr−1.
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