Sentence examples for using a dilution range from inspiring English sources

PCR amplicons were prepared for cycle sequencing by diluting them with water using a dilution range of 1 3 to 1 6, depending on the reaction yield, as determined by agarose gel electrophoresis.

These non-rRNA-hexamers were tested in VIDISCA by using a dilution range of human echovirus 18 culture supernatant (1 E8–1 E4 copies/ml), a virus harvest of which we established that it contains competitor rRNA.

The metabolites glycolate, glycine, serine, glucose-6-phosphate (G6P), sucrose, glycerate, phosphoenolpyruvate (PEP) and 3-phosphoglycerate (3PGA) were externally calibrated using a dilution series of nine concentration points, ranging from 0.04 ng µL−1 to 166.67 ng µL−1 of a chemically defined mixture of authenticated reference compounds in equal amounts at 1.0 mg mL−1 for each compound [32].

To additionally ensure that the quantified western blot signal is within the linear range of detection, we generated standard curves using a dilution series of the respective ubiquitination reaction, which was incubated for 180 min at 30 °C.

MPN values were calculated for both visible turbidity (indicative of a TVC of aerobic microorganisms) and Salmonella, scored using a three dilution range of five tubes.

We further measured the amplification efficiency associated with these two sizes of GAPDH assays using a broad dilution range (5 Log10s) which showed closed efficiencies with 99.98% for GAPDH-67 and 99.92% for GAPDH-122 (data not shown).

The N-GFP and GFP were quantified on a Coomassie blue-stained SDS-PAGE using ImageJ software and a dilution range of BSA as standards.

Primer pairs were validated using a standard curve over a dilution range 1-10-3 1-10-3 1-10-3lope close to -3.32).

For quantification of transcript abundance, a standard curve was generated using purified rpoD PCR product over a dilution range of known concentrations and rpoD transcript quantity in cDNA samples determined by quantitative real-time PCR was used to estimate the relative amount of template concentrations of the experimental genes.

If the manufacturer recommends a range of dilutions, lab will use a dilution in the middle of the recommended dilution range.

Anti-FN and Anti-CNI antibodies were used to inhibit attachment of tenocytes to FN- and CNI- modified substrates in a dilution range of 1 5000 1 500 and 1 1500 1 250, respectively.