Using a dilution plating assay, the DOX hypersensitivity of the diploid hfi1Δ, nup133Δ and ctk1Δ mutations was clearly suppressed when the corresponding wild type gene was expressed from a selectable plasmid (Fig. 3C).
The candidate bacteria were isolated from the DI2 plate, purified using a dilution-plate method with repeated streaking, and preserved in DI1 for subsequent fermentation.
Colony forming units (CFU) were quantitated using a dilution series plated overnight at 37°C.
Following quantification, nested PCR was conducted in 96 well plates using a dilution that deposited approximately one amplifiable template per 5 wells.
Strains were screened for hydroxyurea (HU) and methyl methane sulfonate (MMS) sensitivity using a similar dilution plating procedure as previously described [40].
The MIC of purified lipopeptide was evaluated by using a microtiter plate dilution assay.
The MIC of antimicrobial peptide was evaluated for different strains by using a microtiter plate dilution assay.
Actinomycetes from the soils had been isolated using standard dilution plate method procedure on Petri plates containing starch nitrate agar medium of the following composition (g/L): starch, 20; KNO3, 2; K2HPO4, 1; MgSO4·7H2O, 0.5; NaCl, 0.5; CaCO3, 3; FeSO4·7H2O, 0.01; agar, 20, and distilled water up to 1 L; then plates were incubated for a period of 7 days at 30°C.
Strains were isolated using the soil dilution plate method [36].
Using a replica plate, the serial dilutions of the suspension were patched in YP plates supplemented with 2% glucose, 2% galactose, 2% ethanol or 2% glycerol.
