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Cells with chromatin condensation were visualized and photographed using a digital fluorescence microscope (Olympus) at 30 min after addition of the staining solution.
NFAT and PKC-α subcellular localization was analysed using a digital fluorescence microscope, and images were captured and recorded using a digital-imaging system (Manzati et al. 2005).
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Image acquisition was performed using a digital inverted fluorescence microscope EVOS-xl microscope (Life Technologies, Carlsbad, CA, USA), using a 4×, 10×, 20× or 40× lens, and standard light microscope.
The fluorescent images of whole brains were obtained using a digital camera attached to a fluorescence stereoscopic microscope (Olympus, SZX10).
Fluorescent images were captured using a digital camera attached to a Leica DM4000B fluorescence microscope (Leica, Wetzlar, Germany) and processed for brightness and contrast using Adobe Photoshop.
Sections were washed once with PBS and fixed with 4% paraformaldehyde and photographed under Zeiss fluorescence microscope using a digital camera.
Image acquisition was performed with a Zeiss Axiophot inverted fluorescence microscope using a digital camera and the Zeiss ZEN software.
Recently, Houston et al. (13) presented a flow cytometer for sorting of cells on the basis of fluorescence lifetime using a digital multi-frequency methodology in a modified commercial flow cytometer.
Fluorescence was visualized using Olympus IX70 (Olympus, Tokyo) fluorescence microscope, and images were recorded using a digital SPOT RT3 CCD camera (Diagnostic Instruments inc., Sterling Heights, MI) using version 4.5 SPOT software.
Morphological changes of cell nucleus were examined in a fluorescence microscope, and they were photographed using a digital color camera DFC 300 FX (Leica, Wetzlar, Germany).
using a digital thermometer.
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