Using a differential hybridization screening, a cotton CCCH gene, GhZFP1, was isolated, which functions through interacting with a dehydration protein and a pathogenesis-related protein to positively regulate both salt tolerance and disease resistance [ 9].
Using a "differential smart screen", (DSS) the spectrum of activity contained in a crude extract is measured at several closely related receptor subtypes.
We derive the planet's spectrum using a differential method.
Of the 11 genes that were not tested using promoter fusions, one (AGL61) had been isolated using a similar differential screen and its promoter activity in the female gametophyte had been described previously [ 7, 9].
With the aim of checking the level of background corresponding to common mRNAs in reverse and forward libraries we carried out a differential screening of subtracted libraries using the PCR select differentially screening kit (Clontech, Madrid, Spain), following the manufacturer's instructions.
A differential screening procedure was used to eliminate the false positives in the subtracted cDNA library.
A differential screening of a cold-related pepper seedling cDNA library, using PCR-amplified subtracted and control probes, was performed previously [ 17].
In that study the authors compared this strategy with a differential screening performed on a non-differential cDNA library.
We reasoned therefore that a differential screen using US Food and Drug Administration (FDA -approved compounds may identiFDA -approvedecompoundsvival factors or specific toxins and may be useful for the therapeutidentifyriveitherufacturing of cellselectiveo and posurvivaln vivo.
We designed a differential screen to identify markers differentially expressed in these two cell populations.
