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Using a different lot of azoxymethane from the original study could result in differences in tumorigenesis (i.e. penetrance, size, invasion, etc).
Additionally, we will note in the "Known differences from the original study" section that we will be using a different lot of AOM, and that AOM is known to have lot-to-lot variation in efficacy.
Three replicates were preformed using a different lot of McrBC.
We will be using a different lot of azoxymethane that used by the original authors.
To assess reproducibility, the same samples were then tested in triplicate one week later by a different technologist using a different lot of master mix [ 7].
To further look at the variability of the system, we analyzed the same four tumor RNAs in a third laboratory (located in Paris), at a time several months after the initial comparison was finished, using a different lot of microarrays and different lots of labeling reagents.
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New aliquots were obtained from the original sample archive and were reprocessed using a different kit lot and sequenced.
Following this, a concentration of 0.5 µg/ml was used for future preparations which provided clear and consistent staining in repeated batches using a different antibody lot (Dataset f).
Duplicate hybridisations were performed for each RNA sample on different days using a different filter from the same manufactured lot.
The same lot of labeling kit was used by both the California and Amsterdam laboratories, while a different lot was used by the Paris laboratory.
"The Internet has changed the marketing mix," Ms. Fields said, particularly among the kinds of men who read FHM, primarily ages 21 to 34. "Because you've got a lot of them using a lot of different media, we weave together all these elements.
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