Within each selected pen, 10% of all cattle were enrolled at initial processing using a different, individual animal randomization table.
To verify the results of the array-based methylation analysis, we arbitrarily selected four genes, which were DM on the tiling arrays in either parents or offspring, FUCA1, PCDHAC1, TXNDC16, and RUFY3, and replicated the findings for those, using a different technique and a different animal material.
The second one, using a different group of animals, determined the serum protein changes during the peripartum period of four weeks before, and two-three weeks after lambing.
Using a different injection schedule for Tr animals, i.e. animals were injected during the first three sessions (Tr3 group) or the last four sessions (Tr4-7); we studied if learning promotes survival of the cell generated through acquisition and in which phase of learning occurred.
Moreover, a recent study using a different protocol also demonstrated that animals forcefully exposed to the white compartment three consecutive times presented substantial differences in the behavioral repertoire observed in the light/dark tank [29].
A replication in the outbred HS animals, using a different array platform and population, showed an additive effect of genotype on expression.
Similar findings have been obtained from in vivo animal studies using a different p38 MAPK inhibitor, SB203580 (Calbiochem) which protected mice from endotoxic shock following administration of a lethal dose of LPS [8].
In a second meta-analysis using a different set of studies (k = 5), animal cruelty was found to correlate as well with non-violent offending as it did with violent offending in male and mixed gender samples.
Moreover, both studies used a different reference animal and the reference animal was also from the same breed.
