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We quantified the effect of Mrs1 binding using a difference plot in which the hydroxyl radical cleavage intensities for the free RNA were subtracted from those for the Mrs1-bound RNA (lower panel, Figure 1A).
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In order to better illustrate the minor fluorescence changes between the wild type and mutants, a difference plot was generated using the HRM software.
Figure 1 shows a difference plot of the HRMA data using WT DNA as a baseline, and the corresponding electropherograms.
It was readily apparent that template mixtures containing different ratios for α- and β tryptases gave distinct profiles using the difference plot of the melt curve analysis using a β-tryptase only template as a reference (Fig. 2a).
Using a distribution plot of differences in BW between the two registers, we identified outlying values with large discrepancies (>500 g).
The differences between the clusters were investigated using a contribution plot, which showed the differences in scaled units between the highlighted groups for all variables in the model.
Salanti et al. [ 5] graphically analysed consistency using a forest plot of the differences between direct and indirect evidence in single network loops.
Examination of effects using a forest plot of standardized mean differences where data were available indicated that there was no consistent direction of effect with QoL measures (Fig. S2A GG).
Examination of effects using a forest plot of standardized mean differences where data were available indicated that although many of the studies found no effect on depressive symptoms, mood or anxiety, and the confidence intervals were generally wide they are tending toward a positive effect (Fig. S3A– C).
We visualized protections from hydroxyl radical cleavage, induced upon Mrs1 binding, again using quantitative difference plots (red columns, Figures 1B,C).
Results of the meta-analysis will be reported using a forest plot of the overall standardised mean difference.
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CEO of Professional Science Editing for Scientists @ prosciediting.com