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FTIR ATR spectra were collected on a Perkin Elmer spectrometer 400 equipped with an ATR (attenuated total reflectance) detector using a diamond cell in the wavelength range of 4000 650 cm−1, at a spectral resolution of 4 cm−1 over 32 scans.
FTIR-microscopy studies were performed using a Nicolet Magna-IR 870 bench [ 1] and a Thermo Spectra-Tech Continuumicroscopepe using a diamond cell (Thermo Spectra-Tech, Diamond windows) to avoid exposing the moist protein powders to the high pressure encountered during the formation of KBr pellets usually employed in such studies [ 13, 33].
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The eutectic temperature in the Fe Fe3S system was measured up to 180 GPa using a diamond anvil cell (DAC) (e.g., Kamada et al. 2010, 2012; Morard et al. 2008).
Both the CVD and HPHT processes are also used to create designer optically transparent diamond anvils as a tool for measuring electric and magnetic properties of materials at ultra high pressures using a diamond anvil cell.
Pressure was applied using a diamond anvil cell with methanol/ethanol as the hydrostatic medium and ruby fluorescence as the pressure calibrant.
Embedded pellets of R. sphaeroides cells were serially sectioned with an EM-UC6 ultramicrotome (Leica Microsystems) using a diamond knife (Diatome-US, Hatfield PA).
The embedded cells in blocks were sectioned using a diamond knife on a Leica Ultracut R microtome and ultrathin sections of 90 nm were collected onto pioloform-coated 100 mesh hexagonal copper grids.
This was done using a diamond bur handpiece.
After completing the experiments, the probes were cut sagittally by using a diamond band saw with a 0.3-mm diamond saw blade.
These units were then transected sagittally by using a diamond band pathology saw (EXAKT 312 saw; EXAKT diamond cutting band 0.1 mm D64; EXAKT Advanced Technologies GmbH), generating two identical parts.
Ultra-thin sections were prepared using a diamond knife and a Leica Ultracut R Ultramicrotome.
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